While automatic annotation provides rapid analysis of genes within a sequenced genome, manual annotation results in more accurate analysis. The following proteins are the product of the manually annotated genes from Brachybacterium faecium: orotate phosphoribosyltransferase (E.C. 2.4.2.10), orotidine-5'-phosphate decarboxylase (E.C. 4.1.1.23), dihydroorotase (E.C. 3.5.2.3), aspartate carbamoyltransferase (E.C. 2.1.3.2), and carbamoyl phosphate synthase large and small subunits (E.C. 6.3.5.5). Manual annotation of these genes, using tools from the Annotation Collaboration Toolkit, such as BLAST, T-Coffee, and WebLogo, confirmed the validity of the automatically annotated roles of these genes encoding enzymes that function in the uridine monophosphate (UMP) synthesis pathway.
